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Ortholog conservation Domain conservation Secondary structure 3D structure More Home

Analysis of p.(Thr1927Met) variant, VLGR1 gene, vlgr1 protein (6306 residues)

• Ortholog conservation:

  
  
  

  Number of sequences	AAPI*	AAPIR**	Number of divergencies	Number of mutant	Number of gaps	Conservation of T1927	Conservation - gap
  20	65.35%	60.23%	13 details	1 details	0 details	7 / 20 (35.00%)	7 / 20 (35.00%)

  
  
  

  Divergencies:

  Cavia porcellus - K	Mus musculus - R	Rattus norvegicus - R	Equus caballus - K	Bos taurus - K
  Monodelphis domestica - L	Ornithorhynchus anatinus - M	Anolis carolinensis - R	Gallus gallus - V	Taeniopygia guttata - V
  Xenopus tropicalis - R	Takifugu rubripes - R	Oryzias latipes - K

  Species having the mutant residue:

  Ornithorhynchus anatinus - M

  
  

  There is no gap in the alignment.

  *AAPI: Alignment Average Percentage Identity
  **AAPIR: Alignment Average Percentage Identity of the Region (20 residues surrounding position 1927). AAPIR appears in green if it is more than 10% compared to AAPI, in red if less than 10%.

  You can check the AAPIRs compared with AAPI of the whole alignment by clicking here. The help page will tell you more.

  Display alignment

  Click on the alignment to zoom in and out.

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  Homo sapiens

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  Pan troglodytes

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  Pongo pygmaeus

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  Macaca mulatta

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  Cavia porcellus

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  Mus musculus

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  Rattus norvegicus

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  Canis familiaris

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  Equus caballus

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  Bos taurus

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  Monodelphis domestica

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  Ornithorhynchus anatinus

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  Anolis carolinensis

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  Gallus gallus

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  Taeniopygia guttata

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  Xenopus tropicalis

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  Danio rerio

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  Takifugu rubripes

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  Oryzias latipes

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  Gasterosteus aculeatus

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  Species color legend (basic classification):

  Great apes | Other monkeys | Prosimians | Other mammals | Lizards | Birds | Amphibians | Fishes | Insects | Nematods | Tunicates | Echinoderms

  Show Venn diagram of alignment

  Display WebLogo for the region

  Click on the top-left corner to enlarge.

  

  Display alignment phylogenetic tree

  Click on the top-left corner to enlarge.

  
  

  Download tree in Newick format (right-click on the link, and choose 'Save target as').

  Display region alignment (21 residues, Fasta format)

  Display methods

  Ortholog sequences have been selected from the Ensembl⁽¹⁾ and NCBI websites. Alignment has been performed with ClustalW⁽²⁾, version 1.83 or 2.0.7. Trees have been built using Phylogeny.fr ⁽³⁾, based on the alignments. Software used is PhyML 3.0 aLRT with default parameters. Pictures of trees have been made using Phylip at Mobyle. AAPI and AAPIR have been calculated thanks to Bioperl. WebLogos are drawn with WebLogo 3⁽¹⁴⁾

  Accession numbers

• Domain conservation:

  The residue belongs to the domain Calx-beta 13.

  1911

  1941

  	Calx-beta domains are present as tandem repeats in vlgr1. Their role is unknown.

  
  
  
  
  

  There are no alignments available concerning the domain Calx-beta of the protein vlgr1 or the residue is not present in the alignment.

• Secondary structure analysis:

  Residu T1927 is predicted to belong to a β strand. Probability is 0.712.

  Direct environment is as follow:

  E	I	G	Q	T1927	S	A	N	I

  
  
  

  Observed frequencies in β strands:
  T: 1.21
  M: 1.04

  Mutant residu is less observed in this type of structure.

  Display methods

  Predictions of secondary structures have been made with PsiPred⁽⁹⁾ , version 2.5, using Protein Multiple Sequences Alignments as input, in order to increase the accuracy of the prediction. Amino acid frequencies have been calculated from a non redondant set defined by the RCSB. Details are provided here.

• 3D analysis:

  Models provided and analysed by USMA must be considered as predictions, therefore be careful when interpreting the results. All efforts have been made to build structures of quality, however, they are provided with NO WARRANTY as to their accuracy with the real biological molecules studied.

  Predicted wild type and mutant structures have been compared. You will find the results below. Please note that USMA's "3D engine" is unable to analyse interactions with non amino-acids molecules (e.g. ATP or Ca²⁺).

  • Quick assessment of the overall quality of the structure:
    
    

    PDB template	Sequence identity*	Molprobity bad rotamers	Molprobity Ramachandran outliers	Molprobity Ramachandran favored
    3GIN	33.8 %	1/57 - 1.8 %	2/66 - 3.8 %	62/66 - 93.9 %

    * between target and template

    Otherwise, see detailed Molprobity output

    
    
  • Download wild-type model (PDB format) or mutant prediction.
    
    
  • Check 3D coverage of vlgr1.
    
    
  • This model is made of 0 α helices and of 2 β strands.
    
    
  • 3D model predicts T1927 to be located in a loop (which differs from PsiPred prediction) and M1927 in a loop.
    
    
    
  • The wild type residue is predicted to be exposed and the mutant residue to be exposed.
    
    
  • Hydrogen bond network:
    
    

    T1927	M1927
    none	none

    
    
    
  • The mutant residue is not predicted to introduce steric clashes.
    
    

    T1927	M1927
    none	none

    
    
    
  • Pictures:
    Click on the top-left corner to enlarge.
    Legend: non-covalent bonds/steric clashes: - - - - colors: - - - -: h-bonds, - - - -: ionic bonds, - - - -: hydrophobic interactions; - - - -:clashes.
    
    
    
    
  • If you are not satisfied with the pictures above, or just want to investigate further the structures, you can display the JSmol applet of the wild-type (left) and mutant (right) structures.
    
    Click on the JSmol applet's link to hide it.
    You have a full access to Jmol commands with a simple right click on one applet.
    
    
    
    
    

    T1927 (wild-type)	M1927 (mutant)

    
                  Spin on/off       Sharper/Faster              Change background             

    
    JSmol Legends:
    The residue at the position 1927 is located in the center, labelled in yellow and surrounded by its neighboring residues (distance < 5 Å).
    Amino acids involved in H-bonds with the residue 1927 are labelled in blue.
    Amino acids involved in steric clashes with the residue 1927 are labelled in red.

    Display methods

    All 3D structures except two for harmonin and sans, and myosin VIIa and sans used in USMA are predictions. They are build by homology, (more), using Domain Fishing⁽¹⁵⁾ or Atome 2⁽²⁵⁾ for template recognition, modeller⁽¹⁶⁾ for model building and mutation, and PyMOL⁽¹⁷⁾ and Jmol⁽¹⁸⁾ for visualisation. Each structure has been assessed with MolProbity⁽¹⁹⁾. Msms⁽²⁰⁾ is used to calculate solvent accessibility, and STRIDE⁽²¹⁾ (plus stride2pdb) for secondary structure assignment. When necessary, SCWRL4⁽²²⁾ was used to refine side chains conformations.
    Secondary structure analyses in 3D models uses side chain interaction energies reviewed in ⁽²³⁾, as well as amino-acids propensities for N-caps, N1-N3, helix middle, C3-C1 and C-caps extracted from ⁽²⁴⁾(PDB values).

• Additional ressources:

  
  
  
  • UNIPROT annotations
    

    The variation is not reported in Uniprot.

  • Click on the LOVD picture to check if a variant is described at position 1927    
    
    
    
    
  • Graphical display of the region at NCBI (including SNPs)

See accession numbers of sequences used or references.

Execution time: 1 wallclock secs ( 0.10 usr 0.01 sys + 0.76 cusr 0.32 csys = 1.19 CPU)